Electron transfer of horseradish peroxidase entrapped in poly-o-phenylenediamine membrane electrode
- 化学化工－已发表论文 
The poly-o-phenylenediamine membrane electrode containing horseradish peroxidase was prepared by means of the electrochemical immobilization of enzyme, and the voltammetric behavior of the resulting enzyme electrode and its bioelectrocatalysis to hydrogen peroxide reduction was studied. It was found that the direct electron tansfer between the enzyme and the polymer matrix takes place in the biocatalytic processes, For the newly prepared enzyme electrode, however, the oligomers, which are formed during the polymerization of o-phenylenediamine and then incorporated in the enzyme membrane, can play a role of electron transfer mediators to accelerate the regeneration of the oxidized enzyme, From the fitting of experimental current response curves, it can be found that the change in the regeneration rates of the oxidized enzyme with the electrode potentials obeys apparantly the Tafel relationship. The procedure in the measurement of kinetic parameters for enzyme reactions was suggested.