Fluorescence enhancing by alkaline degradation of tetracycline antibiotics and its application
- 化学化工－已发表论文 
Tetracycline antibiotics could be degraded after being heated in boiling-water bath in alkaline solution (in KOH medium). The fluorescence excitation and emission wavelengths of the degradation products changed and the fluorescence intensity increased by 5-190 folds as compared with that of antibiotics themselves. It was considered that there occurred a nucleophilic ring rupture reaction to form the degradation products with lactonic structure. The inter action of the lone pair electrons of oxygen on the degradation products with the conjugated pi electrons induced the increase of the delocalization for the conjugated electron system in the molecule and thus enhanced the fluorescence intensity.