H-2-uptake activity, spectra, reduction potentials, and kinetics of iron release on the surface of iron core from Azotobacter vinelandii bacterial ferritin
Date
1998Author
Huang, HQ
黄河清
Xu, LS
Zhang, FZ
Qiu, XH
Lin, QM
Huang, JW
Zao, H
Huang, NC
Zeng, RY
Zeng, D
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- 化学化工-已发表论文 [14469]
Abstract
Bacterial ferritin from Azotobucter vinelandii (AvBF(o)) has a function in H-2 uptake. The Fe3+ reduction on the surface of the iron core from AvBF(o) is accompanied simultaneously by H-2 uptake, with a maximum activity of H-2 uptake of 450H(2)/AvBF(o). A reduction potential of -402 mV for iron reduction on the surface of the core is found. A shift to the red the protein absorbance peaks ranging from 280 to 290 nm is observed between pH 5 and 9 under 100% H-2 reduction. The reduction potential for iron release becomes negative at a rate of 0.025 mV/Fe2+ released. The kinetics of iron release on the surface of the core is a first-order reaction.