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H-2-uptake activity, spectra, reduction potentials, and kinetics of iron release on the surface of iron core from Azotobacter vinelandii bacterial ferritin

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H-2-uptake activity, spectra, reduction potentials, and kinetics of iron release on the.htm (400bytes)
Date
1998
Author
Huang, HQ
黄河清
Xu, LS
Zhang, FZ
Qiu, XH
Lin, QM
Huang, JW
Zao, H
Huang, NC
Zeng, RY
Zeng, D
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  • 化学化工-已发表论文 [14469]
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Abstract
Bacterial ferritin from Azotobucter vinelandii (AvBF(o)) has a function in H-2 uptake. The Fe3+ reduction on the surface of the iron core from AvBF(o) is accompanied simultaneously by H-2 uptake, with a maximum activity of H-2 uptake of 450H(2)/AvBF(o). A reduction potential of -402 mV for iron reduction on the surface of the core is found. A shift to the red the protein absorbance peaks ranging from 280 to 290 nm is observed between pH 5 and 9 under 100% H-2 reduction. The reduction potential for iron release becomes negative at a rate of 0.025 mV/Fe2+ released. The kinetics of iron release on the surface of the core is a first-order reaction.
Citation
JOURNAL OF PROTEIN CHEMISTRY,1998,17(1):45-52
URI
http://dx.doi.org/doi:10.1023/A:1022538530701
https://dspace.xmu.edu.cn/handle/2288/13399

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