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dc.contributor.advisor汪德耀
dc.contributor.author李晖
dc.date.accessioned2017-06-20T08:37:14Z
dc.date.available2017-06-20T08:37:14Z
dc.date.issued2017-05-22
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/133746
dc.description.abstract本文通过超微结构观察,多肽成分SDS一聚丙烯酰胺凝 胶电泳研究了下毛类纤毛原生动物厚游仆葱衰老细胞的DNA 复制带的变化,同时探讨了复制带提取方法;并通过核仁超微 结构观察3H—尿苷掺入的光镜及电镜放出自显影比较了衰老 与年轻厚游仆虫的RNA合成变化.主要结果如下: (一)证实衰老厚游仆虫的DNA复制带逐渐变窄消失;发现 复制带中分子量大于67KD的多肽种类呈衰老相关变化;推测 高分子量多肽种类的衰老变化引起复制带的核基质结构改变, 从而导致衰老细胞DNA合成量减少. (二)采用低渗、旋涡振荡、蔗糖溶液离心改进Abben分离 阔口游仆虫复制带的方法而用之于厚游仆虫,首次分离...
dc.description.abstractAge-correlated changes in DNA replication bands (RBS) of Euplotes crassus were investigated by electron microscopy of RBs and SDS-PAGE of protein from RBs, and a method was described in this study for isolating RBs from the cell. Age-dependent changes in RNA symthesis in the macronucleus were also studied by analysis of nucleolar changes and by light and electron microscope autoradiograph ...
dc.language.isozh_CN
dc.relation.urihttp://210.34.4.28/opac/openlink.php?strText=1411&doctype=ALL&strSearchType=callno
dc.source.urihttp://210.34.4.13:8080/lunwen/detail.asp?serial=6074
dc.title厚游仆虫(Euplotes crassus Dujardin)衰老细胞的大核DNA复制带、RNA合成量变化研究
dc.title.alternativeStudies on age related change in DNA replication band and RNA synthesis inthe macronucleus of Euplotes crassus
dc.typethesis
dc.date.replied1991-07-02
dc.description.note学位:理学硕士
dc.description.note院系专业:生命科学学院_细胞生物学
dc.description.note学号:YL000132


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