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dc.contributor.authorZhang, HM
dc.contributor.authorGuo, XQ
dc.contributor.author郭祥群
dc.contributor.authorZhao, YB
dc.contributor.author赵一兵
dc.contributor.authorWang, DY
dc.contributor.authorXu, JG
dc.contributor.author许金钩
dc.date.accessioned2012-07-30T01:59:15Z
dc.date.available2012-07-30T01:59:15Z
dc.date.issued1998-10-15
dc.identifier.citationANALYTICA CHIMICA ACTA,1998,361(1-2):9-17zh_CN
dc.identifier.issn0003-2670
dc.identifier.urihttp://dx.doi.org/10.1016/S0003-2670(98)00009-9
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/13371
dc.description.abstractA novel spectrofluorimetric method has been proposed for the determination of nucleic acids based on shifting the dimer-monomer equilibrium of the fluorescent dye, acridine yellow (AY). Induced by the premicellar aggregation of an anionic surfactant, sodium dodecyl sulphate (SDS), AY formed a nonfluorescent homodimer (AYAY) which largely quenches the fluorescence intensity of the dimer system. However, the fluorescence intensity of the system increased dramatically when nucleic acids were added to the solution. The fluorescence enhancement is probably based on the DNA modulated shift of the dimer-monomer equilibrium of AY in the anionic surfactant solution. Intercalation of the monomer in DNA caused the dissociation of AYAY. and led a very high fluorescence enhancement. A linear dependence of fluorescence intensity on Calf thymus (CT) DNA concentration over the rang of 0 similar to 40 mu g/ml CT DNA allowed sensitive quantification of CT DNA by a simple fluorescent method. The detection limit was 0.023 mu g/ml and the RSD was 1.5% for 2 mu g/ml CT DNA (n=7). Furthermore, calibration graphs for yeast RNA, polynucleotides, such as poly (A) and poly (I) were also obtained. (C) 1998 Elsevier Science B.V.zh_CN
dc.language.isoenzh_CN
dc.publisherELSEVIER SCIENCE BVzh_CN
dc.subjectdimerzh_CN
dc.subjectacridine yellowzh_CN
dc.subjectanionic surfactantzh_CN
dc.subjectnucleic acidszh_CN
dc.subjectfluorescent probezh_CN
dc.titleStudy on the dimer-monomer equilibrium of a fluorescent dye and its application in nucleic acids determinationzh_CN
dc.typeArticlezh_CN


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