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dc.contributor.advisor徐金森
dc.contributor.author吴俊杰
dc.date.accessioned2017-06-20T08:36:58Z
dc.date.available2017-06-20T08:36:58Z
dc.date.issued2016-01-14
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/133665
dc.description.abstract葛根为豆科植物野葛或粉葛的干燥根,为常用中药之一,具有解肌退热、生津、透疹、升阳止泻的功效,多用于治疗外感发热、头痛、项强、消渴、麻疹不透、热痢、泄泻、高血压等症状。葛根素(Puerarin,PU)是葛根的主要活性成分,属于黄酮类化合物,药理作用广泛,是一种很有开发前景的药物。 本实验采用杂交瘤技术制备葛根素的单克隆抗体,在此基础上建立葛根素的酶联免疫吸附分析法检测技术,对葛根中药及中成药中葛根素的含量进行检测和评价。 主要的实验结果如下: (1)采用高碘酸钠法合成免疫人工抗原PU-BSA和包被人工抗原PU-HSA,经MALDI-TOF-MS鉴定,载体蛋白连接的半抗原数分别为18和16个...
dc.description.abstractPueraria is the dry root of Pueraria lobata (wild) Ohwi or Pueraria thomsonii Benth, one of characteristic traditional Chinese medicines, has many pharmaceutical effects, such as anti-inflammatory, reducing blood sugar, blood pressure, and so on. Pharmaceutical researches indicated that puerarin, an isoflavone, is the major pharmaceutical ingredient of herbal Pueraria, functioning lots of bio-medi...
dc.language.isozh_CN
dc.relation.urihttp://210.34.4.28/opac/openlink.php?strText=48563&doctype=ALL&strSearchType=callno
dc.source.urihttp://210.34.4.13:8080/lunwen/detail.asp?serial=37633
dc.subject葛根素
dc.subject单克隆抗体
dc.subject竞争ELISA法
dc.subjectHPLC法
dc.subjectPuerarin
dc.subjectMonoclonal Antibody
dc.subjectCompetitive ELISA
dc.subjectHPLC
dc.title葛根素单克隆抗体的制备及其应用
dc.title.alternativePreparation and Application of Monoclonal Antibody against Puerarin
dc.typethesis
dc.date.replied2012-11-16
dc.description.note学位:理学硕士
dc.description.note院系专业:生命科学学院_细胞生物学
dc.description.note学号:21720091152188


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