Show simple item record

dc.contributor.authorZhu, QZ
dc.contributor.authorZheng, XY
dc.contributor.authorXu, JG
dc.contributor.author许金钩
dc.contributor.authorLi, WY
dc.contributor.authorLiu, FH
dc.date.accessioned2012-07-29T01:25:29Z
dc.date.available2012-07-29T01:25:29Z
dc.date.issued2006-08-23
dc.identifier.citationANALYTICAL LETTERS,1998,31(6):963-971zh_CN
dc.identifier.issn0003-2719
dc.identifier.urihttp://dx.doi.org/doi:10.1080/00032719808002834
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/13365
dc.description.abstractA novel mimetic enzyme immunoassay method has been developed for the determination of hepatitis B surface antigen (HBsAg) in solution. Hemin, a horseradish peroxidase substitute, was used as a labeling reagent to catalyze the reaction of p-hydroxyphenyl acetic (HPA) and hydrogen peroxide in alkaline medium. In the sandwich immunoassay, anti-HBsAg antibody was coated on a 96-well plate (polystyrene), which first reacted with standard HBsAg solution or HBsAg in the test blood serum and then further reacted with the fixed amount of hemin-labelled anti-HbsAg. After the two-step immunoreaction, the immunochemically adsorbed hemin-anti-HBsAg conjugate moiety was determined by measuring the fluorescence produced in a solution containing HPA and hydrogen peroxide. The fluorescence intensity was directly proportional to the concentration of HBsAg. The calibration graph for HBsAg was linear over the range 0-450 ng/well with a detection limit of 0.9 ng/well. The method is sensitive enough for the determination of HBsAg level in hepatitis B blood serum.zh_CN
dc.language.isoenzh_CN
dc.publisherMARCEL DEKKER INCzh_CN
dc.subjectmimetic enzyme immunoassayzh_CN
dc.subjectspectrofluorimetric determinationzh_CN
dc.subjectheminzh_CN
dc.subjectHepatitis B Surface Antigenzh_CN
dc.titleApplication of hemin as a labeling reagent in mimetic enzyme immunoassay for hepatitis B surface antigenzh_CN
dc.typeArticlezh_CN


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record