The determination of creatinine in human urine by capillary zone electrophoresis with photodiode array detection
Date
2006-08-22Author
Jia, L
Gao, J
Chen, X
陈曦
Hu, GL
Wang, YR
Wang, XR
王小如
Zhuang, ZX
庄峙厦
Collections
- 化学化工-已发表论文 [14469]
Abstract
The application of capillary zone electrophoresis (CZE) to the separation and determination of creatinine in human urine with photodiode array detection was described. Optimum conditions for the separation were established. The effect of pH of the background buffer on the existence state of creatinine was investigated. Photodiode array detection permitted the rapid identification of creatinine in the samples analysed. By selecting 234 Mn as monitoring wavelength, the interference from urea in urine was eliminated. By selecting a suitable pH, the interferences from other substances in urine were also eliminated. The linear range, detection limit, precision and recovery of the method were investigated.