Studies on the application of synthesized near-IR cyanine to the determination of trace amounts of protein
- 化学化工－已发表论文 
A new water-soluble near-IR cyanine dye was synthesized and its structure was characterized by H-1 NMR, MS and IR spectra. The dye was used as a probe to establish a novel near-IR spectrophotometric method for the determination of trace amount of serum protein. The dye has a maximum absorption wavelength at 776 nm, its absorbance decreases when it reacts with protein(for example, BSA). The reaction of the dye with BSA was carried out in 0.02 mol/L HAc-NaAc buffer system(pH 4.0). When 3.0x10(-6) mol/L cyanine was used, the linearity of this method was over the range of 1.00 x 10(2)-1.90 x 10(3) ng/mL by determination of HSA and gamma-globulin, and the detection limit was 50 ng/mL.