Determination of glucose based on the effect of photons as a substitute for glucose oxidase

Abstract

Glucose can produce H2O2 in solution when irradiated with UV light even in the absence of glucose oxidase. Based on this effect, photons were utilized as a substrate for glucose oxidase, and a new fluorescent method was developed for indirect determination of glucose by a coupled fluorescence reaction of p-hydroxyphenyl acetic acid and H2O2 with hemin as a catalyst. The calibration graph is linear over the range 0-6.0 x 10(-7) mol/l glucose, the detection limit is 1.7 x 10(-8) mol/l glucose. The method has been applied to the determination of glucose in human sera with satisfactory results. (C) 1999 Elsevier Science B.V. All rights reserved.