Determination of glucose based on the effect of photons as a substitute for glucose oxidase
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Glucose can produce H2O2 in solution when irradiated with UV light even in the absence of glucose oxidase. Based on this effect, photons were utilized as a substrate for glucose oxidase, and a new fluorescent method was developed for indirect determination of glucose by a coupled fluorescence reaction of p-hydroxyphenyl acetic acid and H2O2 with hemin as a catalyst. The calibration graph is linear over the range 0-6.0 x 10(-7) mol/l glucose, the detection limit is 1.7 x 10(-8) mol/l glucose. The method has been applied to the determination of glucose in human sera with satisfactory results. (C) 1999 Elsevier Science B.V. All rights reserved.