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Determination of glucose based on the effect of photons as a substitute for glucose oxidase

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Determination of glucose based on the effect of photons as a substitute for glucose oxidase.htm (413bytes)
Date
1999-04-22
Author
Chen, M
Cai, WP
Zhu, QZ
Wang, XS
Xu, JG
许金钩
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  • 化学化工-已发表论文 [14469]
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Abstract
Glucose can produce H2O2 in solution when irradiated with UV light even in the absence of glucose oxidase. Based on this effect, photons were utilized as a substrate for glucose oxidase, and a new fluorescent method was developed for indirect determination of glucose by a coupled fluorescence reaction of p-hydroxyphenyl acetic acid and H2O2 with hemin as a catalyst. The calibration graph is linear over the range 0-6.0 x 10(-7) mol/l glucose, the detection limit is 1.7 x 10(-8) mol/l glucose. The method has been applied to the determination of glucose in human sera with satisfactory results. (C) 1999 Elsevier Science B.V. All rights reserved.
Citation
ANALYTICA CHIMICA ACTA,1999,388(1-2):11-17
URI
http://dx.doi.org/10.1016/S0003-2670(99)00130-0
https://dspace.xmu.edu.cn/handle/2288/13254

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