基于生物质能源的杜氏藻海水室外周年养殖

Abstract

[中文文摘]将涨潮海水浓缩后以次氯酸钠进行消毒,添加少许氮、磷等无机盐,在室外周年养殖杜氏藻及其多个突变株,研究其在自然条件下生物量的产出和细胞内脂类的积累。结果表明,在养殖过程中,培养液的盐度、pH值以及细菌含量的变化对杜氏藻的生长没有显著影响。通过培养,不同株系杜氏藻都可以获得较高的生物量和脂类产出,特别是耐高温突变株、高脂突变株可以适应夏天室外的高温、高盐等恶劣环境,普通株在冬天尚能生长,不同特性的藻株的轮换养殖可以保证养殖场周年养殖。海水养殖有望成为一种大规模培养杜氏藻的既简单又经济的发展模式。文章为进一步开发利用杜氏藻生物质能源奠定了一定的基础。[英文文摘]Dunaliella bardawil and several mutants were cultured outdoor in concentrated seawater treated by Javel water with the addition of few nitrate and phosphate,and the biomass and lipid accumulation of Dunaliella were assessed.Dunaliella species grew well during the cultivation period,despite of the change of salinity,pH and bacteria content in seawater.High lipid and biomass productivity of Dunaliella species was obtained in our study.High temperature tolerance mutant and high lipid content mutant grew well under high temperature and high salinity of seawater in summer, while normal strain grew well in winter. Alternate cultivation of different Dunaliella strain should be carried out in all-year outdoor cultivation. It would be a simple, economic batch mode for large-scale Dunaliella cultivation using concentrated seawater, and it would contribute to further using of Dunaliella for biomass energy.