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dc.contributor.advisor王德祥
dc.contributor.author黄丹
dc.date.accessioned2017-06-20T08:16:43Z
dc.date.available2017-06-20T08:16:43Z
dc.date.issued2017-03-27
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/129933
dc.description.abstract海绵动物是古老的多细胞动物,其骨架中最重要的组成成分是硅质骨针。与硅质骨针形成密切相关的酶被称为硅蛋白(silicatein),属于类木瓜蛋白酶半胱氨酸蛋白酶亚家族,具有由丝氨酸(Ser)、天冬酰胺(Asn)和组氨酸(His)组成的催化三分子和特征性的羟基氨基酸簇,主要作用是参与海绵动物硅质骨针的形成,其在离体条件下对于生物硅沉淀的催化作用在仿生学应用上具有重要的应用价值。 研究对象叶片山海绵(Mycalephyllophila,Hentschel1911)隶属多孔动物门(Porifera)、寻常海绵纲(Demospongiae)、繁骨海绵目(Poecilosclerida)、山海绵科(My...
dc.description.abstractAs the most primary multicellular organisms on earth, sponges possess the most novel skeletal elements, siliceous spicules. An enzyme named silicatein, which is considered to be a new member of the papain-like cysteine protease subfamily, is closely related to the formation of siliceous spicules. Silicatien is distinguished by a Ser-Asn-His catalytic triad and characteristic serine cluster, which ...
dc.language.isozh_CN
dc.relation.urihttp://210.34.4.28/opac/openlink.php?strText=55588&doctype=ALL&strSearchType=callno
dc.source.urihttp://210.34.4.13:8080/lunwen/detail.asp?serial=56780
dc.subject多孔动物门
dc.subject叶片山海绵
dc.subject海绵硅蛋白α
dc.subject原核表达
dc.subject活性检测
dc.subjectPorifera
dc.subjectsponge
dc.subjectsilicatein α
dc.subjectprokaryotic expression
dc.subjectactivity assay
dc.title叶片山海绵硅蛋白α基因的克隆、原核表达以及重组蛋白的活性检测
dc.title.alternativeCloning and Prokaryotic Expression of the Silicatein-α Gene in Mycale phyllophila and Activity Assay of the Recombinant Silicatein-α
dc.typethesis
dc.date.replied2016-05-18
dc.description.note学位:理学硕士
dc.description.note院系专业:海洋与地球学院_海洋生物学
dc.description.note学号:22320131151410


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