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dc.contributor.authorLiang, Ying
dc.contributor.authorShi, Song-Lin
dc.contributor.authorLi, Qi-Fu
dc.contributor.authorChen, Lan-Ying
dc.contributor.authorJing, Guang-Jun
dc.contributor.authorTan, Guo-Wei
dc.contributor.authorWang, San-Ying
dc.contributor.author王三英
dc.contributor.authorWu, Fu-Yun
dc.date.accessioned2012-06-24T12:03:54Z
dc.date.available2012-06-24T12:03:54Z
dc.date.issued2011-07-07
dc.identifier.citationJournal of Cellular Biochemistry,Vol 112, Issue 7,pages 1722–1729, July 2011zh_CN
dc.identifier.issn0730-2312
dc.identifier.urihttp://dx.doi.org/doi:10.1002/jcb.23063
dc.identifier.uriWOS:000291616000003
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/12973
dc.description.abstractHeterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1 is involved in the synthesis of RNA. Its expression is up-regulated in many tumor cell lines. In this study, we investigated the distribution of hnRNP A2/B1 in the nuclear matrix, including its co-localization with expression products of related genes. Results from 2-DE PAGE and MS showed that hnRNP A2/B1 is involved with components of nuclear matrix proteins of SK-N-SH cells, and that its expression level is down-regulated after retinoic acid (RA) treatment. Protein immunoblotting results further confirm the existence of hnRNP A2/B1 in the nuclear matrix, as well as its down-regulation after RA treatment. Immunofluorescence microscopy observation showed that hnRNP A2/B1 localized in nuclear matrix of SK-N-SH cells and its distribution regions were altered after RA treatment. Laser scanning confocal microscopy observation showed that hnRNP A2/B1 co-localized with c-Myc, c-Fos, P53, and Rb in SK-N-SH cells. The co-localized region was altered as a result of RA treatment. Our data proved that hnRNP A2/B1 is a nuclear matrix protein and can be up-regulated in human neuroblastoma. The expression and distribution of hnRNP A2/B1 can affect the differentiation of SK-N-SH cells, as well as its co-localization with related oncogenes and tumor suppressor genes. J. Cell. Biochem. 112: 1722-1729, 2011. (C) 2011 Wiley-Liss, Inc.zh_CN
dc.description.sponsorshipNational Natural Science Foundation of China[30871241, 81071669]zh_CN
dc.language.isoenzh_CN
dc.publisherWILEY-BLACKWELLzh_CN
dc.subjecthnRNP A2/B1zh_CN
dc.subjectNUCLEAR MATRIXzh_CN
dc.subjectHUMAN NEUROBLASTOMAzh_CN
dc.subjectDIFFERENTIATIONzh_CN
dc.titleThe Localization of hnRNP A2/B1 in Nuclear Matrix and the Aberrant Expression During the RA-Induced Differentiation of Human Neuroblastoma SK-N-SH Cellszh_CN
dc.typeArticlezh_CN


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