Show simple item record

dc.contributor.authorLi, Zichong
dc.contributor.author李子冲
dc.contributor.authorXu, Limei(Third Inst Oceanog)
dc.contributor.authorLi, Fang(Third Inst Oceanog)
dc.contributor.authorZhou, Qing(Third Inst Oceanog)
dc.contributor.authorYang, Feng(Third Inst Oceanog)
dc.date.accessioned2012-06-24T11:51:46Z
dc.date.available2012-06-24T11:51:46Z
dc.date.issued2011
dc.identifier.citationArchives of Virology,Volume 156, Number 7 (2011)zh_CN
dc.identifier.issn0304-8608
dc.identifier.urihttp://dx.doi.org/doi:10.1007/s00705-011-0954-7
dc.identifier.uriWOS:000292159800003
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/12972
dc.description.abstractWhite spot syndrome virus (WSSV) is a large enveloped virus, but the organization of its envelope proteins remains largely unknown. In the present study, we used blue native polyacrylamide gel electrophoresis (BN-PAGE) and SDS-PAGE in combination with mass spectrometry to analyze the envelope protein complexome of WSSV. Our results show that the viral envelope consists of multi-protein complexes (MPCs). Within them, the envelope protein VP19 exists as a homotrimer, while another major envelope protein, VP28, mainly exists as a homotetramer. The most notable feature is that the majority of MPCs include VP26 and VP24, suggesting that these two proteins might serve as hub proteins to recruit low-abundance proteins to MPCs and play crucial roles in the process of protein complex formation. Furthermore, we found significant evidence for interactions between several low-abundance proteins, such as VP52B/VP38/VP33 and VP12/VP150. The result of this study may promote the further research on WSSV envelope assembly.zh_CN
dc.description.sponsorshipNatural Science Foundation of China[30771640, 40776096]; Earmarked Fund for Modern Agro-industry Technology Research System[nycytx-46]zh_CN
dc.language.isoenzh_CN
dc.publisherSPRINGER WIENzh_CN
dc.titleAnalysis of white spot syndrome virus envelope protein complexome by two-dimensional blue native/SDS PAGE combined with mass spectrometryzh_CN
dc.typeArticlezh_CN


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record