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dc.contributor.authorLi, Fang(SOA, Inst Oceanog 3)
dc.contributor.authorKe, Wei
dc.contributor.author柯伟
dc.contributor.authorYan, Xiumin(Chinese Acad Sci)
dc.contributor.authorXu, Limei(SOA, Inst Oceanog 3)
dc.date.accessioned2012-06-16T11:53:04Z
dc.date.available2012-06-16T11:53:04Z
dc.date.issued2011-07-27
dc.identifier.citationFish & Shellfish Immunology Volume 31, Issue 1, July 2011, Pages 161–163zh_CN
dc.identifier.issn1050-4648
dc.identifier.urihttp://dx.doi.org/doi:10.1016/j.fsi.2011.04.002
dc.identifier.uriWOS:000292407900020
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/12799
dc.description.abstractLack of efficient gene delivery and expression methods is the major obstacle for crustacean research in the cellular level. Here, we reported the construction of an expression vector with a strong promoter from shrimp white spot syndrome virus. This vector could efficiently express foreign genes in the primary culture of crayfish hemocytes with the transfection efficiency between 5 and 10% by electroporation. Our findings provide a method for in vitro gene functional study in primary crayfish cells. (C) 2011 Elsevier Ltd. All rights reserved.zh_CN
dc.description.sponsorshipScientific Research Foundation of Third Institute of Oceanography, SOA[2010017]; National Natural Science Foundation of China[40976100, 30800588, 40776096]; Fujian Science and Technology Project[2009101176]zh_CN
dc.language.isoenzh_CN
dc.publisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTDzh_CN
dc.subjectWhite spot syndrome virus (WSSV)zh_CN
dc.subjectPromoterzh_CN
dc.subjectPrimary culture of crayfish hemocyteszh_CN
dc.subjectElectroporationzh_CN
dc.titleGene transfection and expression in the primary culture of crayfish hemocyteszh_CN
dc.typeArticlezh_CN


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