Gene transfection and expression in the primary culture of crayfish hemocytes
Li, Fang（SOA, Inst Oceanog 3）
Yan, Xiumin（Chinese Acad Sci）
Xu, Limei（SOA, Inst Oceanog 3）
- 生命科学－已发表论文 
Lack of efficient gene delivery and expression methods is the major obstacle for crustacean research in the cellular level. Here, we reported the construction of an expression vector with a strong promoter from shrimp white spot syndrome virus. This vector could efficiently express foreign genes in the primary culture of crayfish hemocytes with the transfection efficiency between 5 and 10% by electroporation. Our findings provide a method for in vitro gene functional study in primary crayfish cells. (C) 2011 Elsevier Ltd. All rights reserved.
出处Fish & Shellfish Immunology Volume 31, Issue 1, July 2011, Pages 161–163