A novel method for the determination of total protein in human serum by near infrared fluorescence recovery

Abstract

A novel fluorometric method has been developed for the determination of total protein in human serum with a new near-IR reagent as a fluorescence probe, based on the fluorescence recovery of the cyanine-CTAB system in the presence of protein. Maximum fluorescence is produced with maximum excitation and emission wavelengths at 765 and 812 nm, respectively. Under optimal conditions, the calibration graphs are linear over the range 0.3-12.0 mug/mL for protein. The detection limit is 70 ng/mL, and the relative standard deviation of six replicate measurements is 1.14% for 6.0 mug/mL protein. The results are satisfactory.