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dc.contributor.authorLiang, DH
dc.contributor.authorSong, LG
dc.contributor.authorQuesada, MA
dc.contributor.authorTian, ZW
dc.contributor.author田昭武
dc.contributor.authorStudier, FW
dc.contributor.authorChu, B
dc.date.accessioned2012-06-14T01:23:08Z
dc.date.available2012-06-14T01:23:08Z
dc.date.issued2000-11-22
dc.identifier.citationELECTROPHORESIS,2000,21(17):3600-3608zh_CN
dc.identifier.issn0173-0835
dc.identifier.urihttp://dx.doi.org/doi:10.1002/1522-2683(200011)21:17<3600::AID-ELPS3600>3.0.CO;2-H
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/12763
dc.description.abstractA new method to introduce the concentration gradient into the capillary has been developed and its application to DNA capillary electrophoresis is presented. The concentration gradient produced by mixing 5% w/v polyacrylamide-co-poly(N-dimethylacrylamide) (PAM-co-PDMA) solution and 1 x Tris/N-tris(hydroxymethyl)methyl-3-amino-propanesulfonic acid/EDTA (TT) + 5 M urea buffer was successfully achieved by using two programmable syringe pumps with strict control of dead volume, flow rate, and pressure balance. This method has the advantages of high stability, reproducibility, and versatility. The column with concentration gradient greatly improved the resolution, especially for the large DNA fragments, due to a decrease in band width broadening with time. A column containing 2-4% w/v gradient in four steps had a longer read length, shorter separation time and better resolution (after 380 base) than that of 4% w/v single concentration polymer solution. The number of steps in the gradient had almost no effect on the performance. The change in the average concentration by relocating the position of the same step gradient, i.e., a combination of different low concentration to high concentration polymer solution ratios, resulted in a different migration time, read length and resolution.zh_CN
dc.language.isoenzh_CN
dc.publisherWILEY-V C H VERLAG GMBHzh_CN
dc.subjectconcentration gradientzh_CN
dc.subjectenhancementzh_CN
dc.subjectaverage concentrationzh_CN
dc.titleFormation of concentration gradient and its application to DNA capillary electrophoresiszh_CN
dc.typeArticlezh_CN


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