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Determination of nucleic acids using rivanol as the fluorescent probe in the presence of SDS

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Determination of nucleic acids using rivanol as the fluorescent probe in the presence of SDS.htm (412bytes)
Date
2011-01-01
Author
Li, WY
Xu, JG
许金钩
He, XW
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  • 化学化工-已发表论文 [14469]
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Abstract
The quantitative method for nucleic acids using rivanol as the fluorescent probe in the presence of SDS was proposed. Under proper conditions, addition of nucleic acids to a mixture of rivanol and SDS resulted in enhanced fluorescence and spectral shifts of rivanol-SDS system. The calibration graph was linear in the range of 0-10.0 mug/ml for CT DNA and 0-9.0 mug/ml for yeast RNA, the limit of detection was 62 ng/ml for CT DNA and 156 ng/ml for yeast RNA. CT DNA could be determined in the presence of 20%(w/w) yeast RNA.
Citation
ANALYTICAL LETTERS,2000,33(15):3183-3194
URI
http://dx.doi.org/doi:10.1080/00032719.2000.10399494
https://dspace.xmu.edu.cn/handle/2288/12747

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