Determination of nucleic acids using rivanol as the fluorescent probe in the presence of SDS
- 化学化工－已发表论文 
The quantitative method for nucleic acids using rivanol as the fluorescent probe in the presence of SDS was proposed. Under proper conditions, addition of nucleic acids to a mixture of rivanol and SDS resulted in enhanced fluorescence and spectral shifts of rivanol-SDS system. The calibration graph was linear in the range of 0-10.0 mug/ml for CT DNA and 0-9.0 mug/ml for yeast RNA, the limit of detection was 62 ng/ml for CT DNA and 156 ng/ml for yeast RNA. CT DNA could be determined in the presence of 20%(w/w) yeast RNA.