In-situ photochemical fluorimetry of tyrosine and its determination
- 化学化工－已发表论文 
Tyrosine can produce fluorescent dimers under irradiation with 275nm UV-light in NH3-NH4Cl buffer. Besed on this fact, a new photochemical fluorimetry in-situ was developed for determination of tyrosine. The linear relationship is over the range of 2.0 x 10(-7) similar to 2.0 x 10(-5) mol/L tyrosine. The detection limit is 3.2 x 10(-9) mol/L and relative standard deviation is 1.7%. This method is simple and rapid, and has better selectivity for other amino-acids. It has been applied to the determination of tyrosine in beer and amino-acid injections. Mechanism of the photochemical reaction was discussed.