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dc.contributor.author张智翔
dc.contributor.author段然
dc.contributor.author敬科举
dc.contributor.author吴意珣
dc.contributor.author卢英华
dc.date.accessioned2012-06-10T22:49:08Z
dc.date.available2012-06-10T22:49:08Z
dc.date.issued2012-03
dc.identifier.citation中国抗生素杂志,2012,37(3):202-206,219zh_CN
dc.identifier.issn1001-8689
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/12705
dc.description作者简介:张智翔,男,生于1985年,在读硕士研究生,主要从事微生物发酵,E-mail: net7@xmu.edu.cn *通讯作者,E-mail: jkj@xmu.edu.cnzh_CN
dc.description.abstract[中文文摘]目的利用组合抗性筛选法选育达托霉素高产菌株。方法以玫瑰孢链霉菌(Streptomyces roseosporus ATCC 11379)为出发菌株,通过在不同浓度梯度的达托霉素和链霉素复合抗性平板上进行抗性筛选。结果筛选到一株高产达托霉素的突变株D1000-S3-2,经摇瓶发酵验证达托霉素发酵单位可达59mg/L,比出发菌株提高了63.8%。结论实验证明组合抗性筛选是一种简单高效筛选方法。[英文文摘]Combined antibiotics resistance screening method was applied to screen daptomycin high-producing strain. Methods Based on the biosynthetic pathway and metabolization mechanism of daptoomycin, combined antibiotics resistance screening method was applied to screen daptomycin high-producing strain from original strain of Streptomyces roseosporus ATCC 11379. Results A mutant strain D1000-S3-2 was obtained. The yield of daptomycin was 59 mg/L in fl ask by the mutant strain, which increased 63.8%, compared to that of the parental strain. Conclusion It was demonstrated that the method provides a fast and effective way of screening S. roseosporus.zh_CN
dc.description.sponsorship基金项目:国家自然科学基金(No. 31071488)zh_CN
dc.language.isozhzh_CN
dc.publisher《中国抗生素杂志》编辑部zh_CN
dc.subject达托霉素zh_CN
dc.subject玫瑰孢链霉菌zh_CN
dc.subject组合抗性筛选zh_CN
dc.subjectDaptomycinzh_CN
dc.subjectStreptomyces roseosporuszh_CN
dc.subjectCombined antibiotics resistance screeningzh_CN
dc.title组合抗性筛选法选育达托霉素高产菌株zh_CN
dc.title.alternativeScreening of high daptomycin-producing strains by combined antibiotics resistance screening methodzh_CN
dc.typeArticlezh_CN


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