Role of Kv1. 3 channel of CD4--+ T lymphocytes in the formation of atherosclerosis in rat spleen
- 医学院－已发表论文 
目的:探讨大鼠动脉粥样硬化(AS)模型中Cd4+T淋巴细胞电压门控钾通道(kV)kV1.3的表达、功能及其在AS中的作用。方法:采用高脂饮食法建立大鼠动脉粥样硬化模型,流式细胞术分析淋巴细胞的比例,采用免疫磁珠法分离Cd4+T淋巴细胞,研究脾组织Cd4+T淋巴细胞kV1.3MrnA表达、细胞内钙离子浓度及细胞因子分泌的变化。结果:(1)AS组脾组织Cd4+T淋巴细胞占总T淋巴细胞比例较对照组明显升高(74.93%±2.15%VS67.80%±2.54%,P<0.05)。(2)经刀豆蛋白A(COnA)刺激,AS组T淋巴细胞增殖程度明显高于对照组(1.1321±0.1750VS0.7971±0.0955,P<0.05)。(3)AS组脾组织Cd4+T淋巴细胞在COnA刺激状态下胞内钙离子浓度明显高于对照组(H=82,P<0.05)。(4)AS组脾组织Cd4+T淋巴细胞在刺激48H后较刺激24H后细胞因子(Il-2,Tnf-α)分泌显著增加。(5)AS组脾组织Cd4+T淋巴细胞kV1.3MrnA表达明显高于对照组(3.670±1.579VS1)。结论:AS组脾组织Cd4+T淋巴细胞比例高于对照组,Cd4+T淋巴细胞kV1.3MrnA表达增多,提示高表达kV1.3的Cd4+T淋巴细胞可能在AS的发生发展中发挥重要的作用。AIM: To investigate the function of voltage-gated potassium channel Kv1.3 and its possible role in CD4 + T lymphocytes in the formation of atherosclerosis ( AS) in rat spleen.METHODS: The rat atherosclerosis model was established by feeding high-fat diet.The proportion of lymphocytes was determined by flow cytometry.The CD4 + T lymphocytes were separated using immunomagnetic bead.The mRNA expression of Kv1.3 in CD4 + T lymphocytes was detected.The concentrations of intracellular calcium and cytokines were also measured.RESULTS: ( 1) The proportion of CD4 + T lymphocytes in AS group was significantly higher than that in control group ( 74.93% ± 2.15% vs 67.80% ± 2.54% ,P < 0.05) .( 2) After stimulated with concanavalin A ( ConA) ,the proliferation of CD4 + T lymphocytes in AS group was significantly higher than that in control group ( 1.1321 ± 0.1750 vs 0.7971 ± 0.0955,P < 0.05) .( 3) After stimulated with ConA,the concentration of intracellular calcium in AS group was higher than that in control group.( 4) In AS group,the releases of cytokines of IL-2 and TNF-α in AS group were significantly higher when stimulated with ConA for 48 h than that for 24 h.( 5) The mRNA expression of Kv1.3 in CD4 + T lymphocytes was greatly higher in AS group than that in control group ( 3.670 ± 1.579 vs 1) .CONCLUSION: In AS rats,the increase in CD4 + T lymphocytes as well as the augmentation of Kv1.3 mRNA expression in the cells suggest that up-regulation of Kv1.3 mRNA expression in CD4 + T lymphocytes may be involved in the mechanism of atherosclerotic formation in rat spleen.