Sensitivity-enhanced experiments for the measurement of J and dipolar coupling constants
- 化学化工－已发表论文 
A sensitivity-enhanced IPAP NMR experiment was described in this paper, which separates the H-1-N-15 doublets into two different spectra to alleviate the problem of resonance overlaps and achieve the accurate measurement of J and residual dipolar coupling constants in proteins. This experiment offered 20%-60% sensitivity enhancement over the original IPAP experiment, and therefore produced more measurable resonances. Pulsed field gradient was used for coherence selection. Water-flip-back approach was used for water suppression. The sensitivity-enhanced IPAP experiment was employed in the measurement of (1)J(NH) and D-1(NH) constants of the protein UBC9.