Determination of nucleic acids based on shifting the association equilibrium between tetrasulphonated aluminum phthalocyanine and alcian blue
- 化学化工－已发表论文 
Based on the ability of nucleic acids to shift the association equilibrium of an ion-association complex of alcian blue and tetra-sulfonated aluminum phthalocyanine, thus leading to an increase in phthalocyanine fluorescence, a new method was suggested for the fluorimetric determination of nucleic acids. Investigations were carried out on the spectral characteristic, selection of the buffer system, effect of pH, influence of reaction time, the usage of reagents and interference of foreign substances. Under optimum conditions, the calibration curves for determinations of calf thymus deoxyribonucleic ( DNA) (CTDNA), salmon DNA (SMDNA) and yeast ribonucleic acid (RNA) were linear. over the range of 2.0 similar to 200 mug/L. The detection limits for. CTDNA, SMDNA and RNA were 2.0 mug/L, 1.8 mug/L and 5.4 mug/L, respectively. This method was used to the determination of golden staplylococuus DNA and the result was in agreement with that achieved by an ultraviolet spectrophotometric method.
CitationCHINESE JOURNAL OF ANALYTICAL CHEMISTRY，2002,30（8）:946-949