Imaging of intracellular acidic compartments with a sensitive rhodamine based fluorogenic pH sensor
- 生命科学－已发表论文 
The parameters of intracellular acidic compartments are altered in apoptosis, autophagy, cancer metastasis, etc. Low background and selective staining of lysosomes and autophagosomes was achieved with N-(rhodamine 6G)-lactam-ethylenediamine (R6G-EDA) which fluoresces in acidic vesicles via pH mediated ring opening of the intramolecular lactam. Long retention time of R6G-EDA in lysosomes and its exceptional stability against photo-bleaching allow full time tracking of lysosome morphology changes in tumor necrosis factor-alpha (TNF-alpha) triggered cell death, suggesting its utility for acidic vesicle studies in cell biology.
CitationAnalyst July.2011, Vol.136, Issue(18):3698-3706