Determination of bioavailable cadmium in environmental samples by IP-RP-HPLC coupled with on-line fluorescence assay
- 化学化工－已发表论文 
A novel reserved-phase ion-pair liquid chromatographic method using hexadecyltrimethylammonium bromide (HTMAB) as an ion-pair reagent for separating the complexes of metal and 8-hydroxyquinoline-5-sulphonic acid (HQS) coupled with on-line fluorescence detection has been developed. The chromatographic behavior of metal-HQS complexes in the proposed system was systematically investigated. When the C-18 column modified by HTMAB and the suitable mobile phase coupled with fluorescence detection (lambda(ex) = 388 nm; lambda(em) = 518 nm) were employed, the detection limit of cadmium is 7. 72 ng/mL with an injection volume of 20 muL, and the linear range of calibration curve for cadmium is 50-800 ng/mL. It was successfully applied to determination of bio-available cadmium in polluted soils of China. Moreover, the possible species of Cd-HQS on the column has been investigated by log-log plot analysis.