• 中文
    • English
  • English 
    • 中文
    • English
  • Login
View Item 
  •   DSpace Home
  • 化学化工学院
  • 化学化工-已发表论文
  • View Item
  •   DSpace Home
  • 化学化工学院
  • 化学化工-已发表论文
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Determination of proteins at nanogram levels by their quenching effect on the chemiluminscence reaction between luminol and hydrogen peroxide with manganese-tetrasulfonatophthalocyanine as a new catalyst

Thumbnail
Full Text
Determination of proteins at nanogram levels by their quenching effect on the chemiluminscence reaction between luminol and hydrogen per.htm (400bytes)
Date
2002
Author
Li, YX
Zhao, DH
Zhu, CQ
Wang, T
Xu, JQ
许金钩
Collections
  • 化学化工-已发表论文 [14469]
Show full item record
Abstract
The manganese-tetrasulfonatophthalocyanine (MnTSPc) catalyzed luminol-hydrogen peroxide chemiluminescence (CL) systems can be quenched in the presence of proteins. A highly sensitive CL quenching method has been developed for the determination of proteins. Under optimum conditions, the linear ranges of the calibration curves were 0.1-20 mug/mL for human serum albumin (HSA), 0.2-20 mug/mL for human gamma-IgG, and 0.5-50 mug/mL for the bovine serum albumin (BSA) with the corresponding detection limits were 1.9 ng/mL, 2.7 ng/mL, and 3.4 ng/mL. The method has been applied to the analysis of total proteins in human serum samples and the results were in good agreement with clinical data provided.
Citation
ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2002,374(3):395-398
URI
http://dx.doi.org/doi:10.1007/s00216-002-1498-1
https://dspace.xmu.edu.cn/handle/2288/12309

copyright © 2002-2016  Duraspace  Theme by @mire  厦门大学图书馆  
About | Policies
 

 

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

LoginRegister

copyright © 2002-2016  Duraspace  Theme by @mire  厦门大学图书馆  
About | Policies