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Determination of nucleic acids at nanogram levels by their enhancement effect on the rate of the metalloporphyrin-catalyzed p-hydroxyphenylacetic acid fluorescence reaction

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Determination of nucleic acids at nanogram levels by their enhancement effect on the rate of the metalloporphyrin-catalyzed p-hydrox.htm (400bytes)
Date
2002
Author
Chen, XL
Li, ZB
Wei, L
Qu, HH
Xu, JG
许金钩
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  • 化学化工-已发表论文 [14237]
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Abstract
Nucleic acids were determined on the basis of the finding that the catalytic activity of iron(III) tetrakis(o-aminophenyl)porphyrin (Fe(III)(o-NH2)TPP), a mimetic peroxidase, in the fluorogenic reaction between p-hydroxyphenylacetic acid (p-HPA) and H2O2 is significantly enhanced in the presence of DNA (or RNA). The degree of fluorescence enhancement is a measure of the nucleic acid concentration. Under optimum conditions, the calibration curves for the determination of calf thymus (CT DNA) and yeast RNA were linear over the ranges 0-200.0 ng mL(-1). he corresponding detection limits are 0.8 ng mL(-1) for CT DNA and 1.1 ng mL(-1) for yeast RNA, respectively. The relative standard deviation of seven replicate measurements is 2.2% for 100 ng mL(-1) CT DNA. Interferences from some interesting co-existing substances in the determination of DNA were also examined. The potential application of the method was also further proved by the determination of DNA in golden staphylococcus DNA sample.
Citation
MICROCHIMICA ACTA,2002,140(3-4):241-246
URI
http://dx.doi.org/doi:10.1007/s00604-002-0907-7
https://dspace.xmu.edu.cn/handle/2288/12303

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