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dc.contributor.authorHuang, Qing-Yu
dc.contributor.authorHuang, Lin
dc.contributor.authorHuang, He-Qing
dc.contributor.author黄河清
dc.date.accessioned2012-05-06T14:11:23Z
dc.date.available2012-05-06T14:11:23Z
dc.date.issued2010-09-03
dc.identifier.citationComparative Biochemistry and Physiology Part C: Toxicology & Pharmacology Volume Jan 2011,153(1): 67–74zh_CN
dc.identifier.issn1532-0456
dc.identifier.urihttp://dx.doi.org/doi:10.1016/j.cbpc.2010.08.009
dc.identifier.uriWOS:000285814600008
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/12300
dc.description.abstractMethyl parathion (MP), an organophosphorus pesticide used worldwide, has been associated with a wide spectrum of toxic effects on organisms in the environment. This study set out to analyze the alteration of protein profiles in MP-exposed zebrafish (Danio rerio) brain and find the proteins responsive to MP toxicity. Zebrafish were subjected to 1, 3 and 5 mg/L MP and the proteomic changes in their brains were revealed using two-dimensional gel electrophoresis. Six protein spots were observed to be significantly changed by MP exposure. Among these, 4 spots were down-regulated, while 2 spots were up-regulated. These altered spots were excised from the gels and identified by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry and database searching. The results indicate that these proteins were involved in binding, catalysis, regulation of energy metabolism and cell structure. These data may provide novel biomarkers for the evaluation of MP contamination and useful insights for understanding the mechanisms of MP toxicity. (C) 2010 Elsevier Inc. All rights reserved.zh_CN
dc.description.sponsorshipState Natural Science Fund[40776060]; 973 Projects, China[2010CB12640]zh_CN
dc.language.isoenzh_CN
dc.publisherELSEVIER SCIENCE INCzh_CN
dc.subjectMethyl parathionzh_CN
dc.subjectZebrafishzh_CN
dc.subjectBrainzh_CN
dc.subjectProteomicszh_CN
dc.subjectAlteration of protein profileszh_CN
dc.subjectBiomarkerszh_CN
dc.titleProteomic analysis of methyl parathion-responsive proteins in zebrafish (Danio rerio) brainzh_CN
dc.typeArticlezh_CN


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