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dc.contributor.authorDeng, X
dc.contributor.authorLi, QB
dc.contributor.author李清彪
dc.contributor.authorLu, YH
dc.contributor.author卢英华
dc.contributor.authorSun, DH
dc.contributor.authorHuang, YL
dc.contributor.authorChen, XR
dc.date.accessioned2012-04-26T01:09:38Z
dc.date.available2012-04-26T01:09:38Z
dc.date.issued2003-03-15
dc.identifier.citationWATER RESEARCH,2003,37(10):2505-2511zh_CN
dc.identifier.issn0043-1354
dc.identifier.urihttp://dx.doi.org/10.1016/S0043-1354(03)00027-7
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/12170
dc.description.abstractThis study constructed a genetically engineered Escherichia coli JM109 which simultaneously expressed nickel transport system and metallothionein to remove and recover Ni2+ from aqueous solution. Bioaccumulation process was rapid and followed linearized Langmuir isotherm. A more than six-fold increase of Ni2+ binding capacity was obtained by genetically engineered E coli cells compared with original host E coli cells. A pH assay showed genetically engineered E coli cells accumulated Ni2+ effectively over a broad range of pH (4-10). The presence of 1000 mg/L Na+ and Ca2+, or 50 mg/L Cd2+ or Pb2+ did not have a significant effect on Ni2+ bioaccumulation, while Mg2+, Hg2+ and Cu2+ posed a severe adverse influence on Ni2+ uptake by genetically engineered E coli. Furthermore, genetically engineered E coli cells did not require extra nutrients for Ni2+ bioaccumulation. (C) 2003 Elsevier Science Ltd. All rights reserved.zh_CN
dc.language.isoenzh_CN
dc.publisherPERGAMON-ELSEVIER SCIENCE LTDzh_CN
dc.subjectEscherichia colizh_CN
dc.subjectnickelzh_CN
dc.subjectbioaccumulationzh_CN
dc.subjectgenetic engineeringzh_CN
dc.titleBioaccumulation of nickel from aqueous solutions by genetically engineered Escherichia colizh_CN
dc.typeArticlezh_CN


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