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dc.contributor.authorLi, B
dc.contributor.authorWang, QQ
dc.contributor.author王秋泉
dc.contributor.authorYan, H
dc.contributor.authorYang, LM
dc.contributor.author杨利民
dc.contributor.authorHuang, BL
dc.contributor.author黄本立
dc.date.accessioned2012-04-21T23:47:16Z
dc.date.available2012-04-21T23:47:16Z
dc.date.issued2003
dc.identifier.citationANALYTICAL AND BIOANALYTICAL CHEMISTRY,2003,376(6):923-927zh_CN
dc.identifier.issn1618-2642
dc.identifier.urihttp://dx.doi.org/doi:10.1007/s00216-003-1966-2
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/12140
dc.description.abstractA novel reversed phase ion-pair micro HPLC system with on-line fluorescence detection has been developed systematically and studied for the determination of cadmium in its bio-available fractions of soil samples. In this system, a micro ODS column of 1.0 mm i.d.x150 mm length and a mobile phase containing 6 mmol L-1 8-hydroxyquinoline 5-sulphonic acid (HQS), 3 mmol L-1 cetyltrimethylammonium bromide (CTMABr), 10 mmol L-1 acetic acid-acetate buffer (pH 5.1) as well as 50% acetonitrile at 50 muL min(-1) flow rate were employed to determine cadmium with a 2 muL flow cell through its fluorescence at 518 nm under 338 nm excitation. Furthermore, the composition of Cd-HQS chelate formed on the column was confirmed to be [Cd(HQS)(2)](2-) through a log-log plot method, and then combined with the ion-pair reagent by the electrostatic force under the chromatographic condition proposed. With such a method, the detection limit of cadmium was 8.48 ng mL(-1) (3sigma) with 1 muL sample injection, and the linear range for the determination of cadmium was 30-800 ng mL(-1) (R-2=0.992). This method has been successfully applied to determination of cadmium in its bio-available fractions of BCR-483 and soil samples without interference from other coexistent metal ions. The RSD (n=6) was less than 7.3%. The results were in agreement with the indicative value for BCR-483 and those for the soil samples obtained by ICP-MS with a pretreatment of bis(1,1,3,3-tetramethylbutyl) phosphinic acid extraction.zh_CN
dc.language.isoenzh_CN
dc.publisherSPRINGER-VERLAG BERLINzh_CN
dc.subjectmicro HPLCzh_CN
dc.subjectbio-available cadmiumzh_CN
dc.subjectelemental fractionationzh_CN
dc.titleChromatographic behavior of cadmium in an ion-pair reversed-phase micro HPLC system and its application to the determination of bio-available cadmium in soil sampleszh_CN
dc.typeArticlezh_CN


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