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dc.contributor.author张国广
dc.contributor.author苏勇波
dc.contributor.author沈明山
dc.contributor.author陈亮
dc.date.accessioned2016-05-17T06:53:21Z
dc.date.available2016-05-17T06:53:21Z
dc.date.issued2010
dc.identifier.citation漳州师范学院学报(自然科学版),2010,(3):102-105
dc.identifier.issn1008-7826
dc.identifier.otherZSXZ201003022
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/119011
dc.description.abstract人角质细胞生长因子(kErATInOCyTE grOWTH fACTOr,kgf)在组织的损伤修复中起着重要的作用.植物细胞生物反应器是一种成本低、安全性好的蛋白生产系统.本研究将人kgf-1经农杆菌介导法转入水稻中,共获得38株抗性再生植株.SOuTHErn杂交和rT-PCr结果表明kgf-1基因整合入水稻基因组中且部分转化株中kgf-1基因得到转录.WESTErn blOTTIng检测结果显示有两株转化水稻中能够表达kgf-1.
dc.description.abstractKeratinocyte growth factor(KGF) plays an important role in the tissue damage repair.Plant cell bioreactor is a low cost and safe protein production system.Human KGF-1 gene was transformed into rice by Agrobacterium-mediated method and a total of 38 putative transgenic rice plants were obainted.The results of southern blotting and RT-PCR assay showed that KGF-1 gene was integrated into the rice genome and KGF-1 gene was transcribed in part of the putative transformed rices.Western blotting results showed that KGF-1 was expressed in two transgenic rices.
dc.description.sponsorship福建省自然科学基金资助项目(2010J01240)
dc.language.isozh_CN
dc.subject角质细胞生长因子
dc.subject水稻遗传转化
dc.subject基因表达
dc.subjectkeratinocyte growth factor-1
dc.subjectrice genetic transformation
dc.subjectgene expression
dc.title人角质细胞生长因子-1在水稻中的表达
dc.title.alternativeRecombinant Keratinocyte Growth Factor-1 Expression in Rice
dc.typeArticle


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