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dc.contributor.author王家庆
dc.contributor.author刘晓慧
dc.contributor.author李代宗
dc.contributor.author宋波澜
dc.contributor.author王志平
dc.contributor.author郭冉
dc.date.accessioned2016-05-17T06:52:47Z
dc.date.available2016-05-17T06:52:47Z
dc.date.issued2009
dc.identifier.citation武汉大学学报(理学版),2009,(3):82-87
dc.identifier.issn1671-8836
dc.identifier.otherWHDY200903017
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/118886
dc.description.abstract腺苷酸转移酶(AnT)是位于线粒体内膜上参与能量分子传导的转运蛋白,在细胞凋亡调控网络中发挥重要作用.以虹鳟鱼(OnCOrHynCHuS MykISS)为研究材料,使用rT-PCr和rACE法分离和克隆了虹鳟鱼AnT2基因CdnA的全长序列(gEnbAnk登录号:fJ591153).该序列全长1 276 bP,其中5′端非翻译区长66 bP,3′端非翻译区长316 bP,开放性阅读框长894 bP,编码297个氨基酸.该蛋白具有3个保守的线粒体穿膜功能结构域和3个转膜区.通过与其他脊椎动物腺苷酸转移酶蛋白序列的比较,发现该基因具有高度保守性,氨基酸序列的同源性均在90%左右.同源模建显示其与牛的线粒体AdP/ATP载体,CHAIn A蛋白有相同的孔洞(CAVITy)结构.
dc.description.abstractThe adenine nucleotide translocase(ANT) family of proteins are the most abundant inner membrane proteins of the mitochondrion.They are responsible for the transport of adenine nucleotides across the inner mitochondrial membrane,importing ADP for oxidative phosphorylation and exporting ATP to the cytosol.In this paper,RT-PCR and rapid amplification of cDNA ends(RACE) methods were used for the isolation of the whole cDNA of ant2 gene(GenBank accession number: FJ591153) from rainbow trout Oncorhynchus mykiss.Sequence analysis reveals a 1 276 bp cDNA containing 66 bp 5′-UTR,316 bp 3′-UTR and 894 bp ORF encoding 297 amino acids.It also has three conserved domains of mitochondrial carrier proteins and three transmembrane helixes.ANT2 shows extensive similarities to the known ADP/ATP translocase polypeptides,and the similarity is about 90% between vertebrates.Homology modeling shows that rainbow trout ANT2 protein has the same cavity structure with the ADP/ATP carrier,chain A of cattle.
dc.description.sponsorship河北省科技厅科技计划项目(0624240011)
dc.language.isozh_CN
dc.subject虹鳟鱼
dc.subject腺苷酸转移酶基因2
dc.subjectRT-PCR
dc.subjectcDNA末端快速扩增
dc.subjectrainbow trout(Oncorhynchus mykiss)
dc.subjectant2
dc.subjectRT-PCR
dc.subjectrapid amplification of cDNA ends(RACE)
dc.title虹鳟腺苷酸转移酶基因2(ant2)cDNA克隆与蛋白结构分析
dc.title.alternativeCloning and Analysis of ant2 Full-Length cDNA Derived from Rainbow Trout(Oncorhynchus mykiss)
dc.typeArticle


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