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dc.contributor.author周显凤
dc.contributor.author张伟
dc.contributor.author黄耀坚
dc.contributor.author郑忠辉
dc.contributor.author沈月毛
dc.date.accessioned2016-05-17T06:52:44Z
dc.date.available2016-05-17T06:52:44Z
dc.date.issued2009
dc.identifier.citation厦门大学学报(自然科学版),2009,(6):105-108
dc.identifier.issn0438-0479
dc.identifier.otherXDZK200906022
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/118870
dc.description.abstract研究不同条件下反相柱层析对去乙酰真菌环氧乙酯分离纯化的影响.通过rP-18中压柱层析对从红树林内生真菌拟茎点霉(PHOMOPSISSP.)A-1-2-3诱变后突变株g1-1固体发酵提取物中的去乙酰真菌环氧乙酯进行了纯化精制.对rP-18柱层析纯化去乙酰真菌环氧乙酯操作条件进行了考察,使去乙酰真菌环氧乙酯的含量从28%左右提高到81.5%左右,回收率大于90%.采用rP-18直接洗脱,溶析结晶,重结晶,结晶残液rP-18分步洗脱的总工艺路线,去乙酰真菌环氧乙酯的总回收率为87.7%,产品最终纯度达到99%以上.
dc.description.abstractThe effects on purification of deacetylmycoepoxydiene(DAM) in different conditions were studied.Preparation of DAM from the crude extract of fermentation of strain G1-1,a mutant strain of mangrove endophytic phomopsis sp.A-1-2-3,was conducted using C18-silica gel reversed phase chromatography at moderate pressure.In the process,the operating conditions of C18-silica gel chromatography were studied experimentally,then the purity of DAM reached about 81.5% from about 28% in the crude extract,and the recovery of DAM was over 90%.The whole process of preparation was as follow: direct elution with RP-18,solvent-out crystallization,recrystallization and segment elution of residue of crystallization with RP-18.Through the whole process flow,the general recovery of DAM was 87.7%,and the purity of DAM reached 99% or higher.
dc.description.sponsorship国家海洋863项目(2007AA091503)资助
dc.language.isozh_CN
dc.subject拟茎点霉
dc.subject去乙酰真菌环氧乙酯
dc.subject柱层析
dc.subject分离纯化
dc.subjectPhomopsis sp.
dc.subjectdeacetylmycoepoxydiene
dc.subjectchromatography
dc.subjectpurification
dc.title反相柱层析制备去乙酰真菌环氧乙酯
dc.title.alternativePreparation of Deacetylmycoepoxydiene with Reversed Phase Chromatography
dc.typeArticle


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