Differential methylation of CpG islands within the dermo1 gene promoter in several cancer cell lines
Toh, Hween-Boon（ Natl Univ Singapore, Yong Loo Lin Sch Med）
Ding, Zhijie（Xiamen Univ, Zhongshan Hosp）
Hong, Liang（Huazhong Univ Sci & Technol）
Chen, Chien-Shing（ Natl Univ Singapore, Yong Loo Lin Sch Med）
- 化学化工－已发表论文 
Inactivation of tumor-related genes by promoter hypermethylation is a common epigenetic event in the development of variety of tumors. Dermal (also called twist2) is a novel cancer-related gene which belongs to the basic helix-loop-helix (bHLH) transcription factor family. Herein, we report that dermal expression was sporadically abrogated in human cancer cells by transcriptional silencing associated with CpG island promoter hypermethylation. Direct sequencing of bisulfite-modified DNA from a panel of seven human cancer cell lines (HL60, Molm14, MV4-11, RS4:11, MDM-BA231, H358, and H1299) revealed that CpG dinucleotides in the dermal promoter were methylated. RT-PCR results demonstrated that dermal CpG island hypermethylation was accompanied by a low basal dermal expression level. Our data implicate dermal as a tumor suppressor gene and a valuable molecular marker for human cancer.