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dc.contributor.authorHuang, He-Qing
dc.contributor.author黄河清
dc.contributor.authorHuang, Fu-Sheng
dc.contributor.authorChen, Hai-Bin
dc.contributor.authorHuang, Qing-Yu
dc.contributor.authorHuang, Lin
dc.date.accessioned2012-03-22T06:54:41Z
dc.date.available2012-03-22T06:54:41Z
dc.date.issued2011-07-05
dc.identifier.citationAppl Biochem Biotechnol. 2011 Oct;165(3-4):978-88.zh_CN
dc.identifier.issn0273-2289
dc.identifier.urihttp://dx.doi.org/doi:10.1007/s12010-011-9313-0
dc.identifier.uriWOS:000296640000020
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/11837
dc.description.abstractAn analytical approach using the two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) technique separated the proteome from the optic ganglia of Octopus vulgaris (OVOG). Approximately 600 protein spots were detected from the extraction when applying 150 mu g protein to a 2D-PAGE gel in the pH range 5.0-8.0. Compared to the control, significant changes of 18 protein spots were observed in OVOG under the stress of native seawater containing 2% methanol for 72 h. Among these spots, we found that eight were down-regulated and ten were up-regulated in the gels, which were further identified using both peptide mass fingerprinting and database searches. Significant proteins such as glyceraldehyde-3-phosphate dehydrogenase, alpha subunit of succinyl-CoA synthetase, alcohol dehydrogenase, and long-chain specific acyl-CoA dehydrogenase were up-regulated proteins, whereas putative ABC transporter was a down -regulated protein. These differential proteins at the level of subcellular localization were further classified using LOCtree software with a hierarchical system of support vector machines. We found that most of the differential proteins in the gel could be identified as mitochondrial proteins, suggesting that these protective or marker proteins might help to prevent methanol poisoning via the mitochondria in the optical ganglia. The results indicated that both beta-tubulin and beta-actin were potential biomarkers as up-regulated proteins for monitoring methanol toxicosis associated with fish foods such as octopus and shark.zh_CN
dc.description.sponsorshipState Natural Science Fund[40776060]; 973 Projects[2010CB126403]; PCSIRT Project[IRT0941]zh_CN
dc.language.isoenzh_CN
dc.publisherHUMANA PRESS INCzh_CN
dc.subjectMethanolzh_CN
dc.subjectBiomarkerszh_CN
dc.subjectProteomicszh_CN
dc.subjectOptic gangliazh_CN
dc.subjectOctopus vulgariszh_CN
dc.titleDifferential Proteins of the Optic Ganglion in Octopus vulgaris Under Methanol Stress Revealed Using Proteomicszh_CN
dc.typeArticlezh_CN


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