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dc.contributor.authorTao, Yi-Ming
dc.contributor.authorXu, Xin-Qi
dc.contributor.authorMa, Su-Juan
dc.contributor.authorLiang, Ge
dc.contributor.authorWu, Xiao-Bing
dc.contributor.authorLong, Min-Nan
dc.contributor.author龙敏南
dc.contributor.authorChen, Qing-Xi
dc.contributor.author陈清西
dc.date.accessioned2012-03-22T06:38:53Z
dc.date.available2012-03-22T06:38:53Z
dc.date.issued2011-09-15
dc.identifier.citationJ. Agric. Food Chem., 2011, 59 (20), pp 10971–10975zh_CN
dc.identifier.issn0021-8561
dc.identifier.urihttp://dx.doi.org/doi:10.1021/jf203712n
dc.identifier.uriWOS:000295915000026
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/11836
dc.description.abstractIn order to optimize the cellulase (from Aspergillus glaucus) hydrolysis of pretreated rice straw, the effects of varying enzyme concentration, temperature, and pH were studied. The best experimental conditions found to degrade the pretreated rice straws were 24 h of incubation at 55 degrees C and pH 5.0, with an enzyme concentration of 48 mg/L. Urea is one of the important nitrogen sources used in fungi culture, but it is also a denaturant. The model of denaturation of endoglucanase (EG) in urea solutions was established. The denaturation was a slow, reversible reaction. Determination of microscopic rate constants showed k(+0) > K'(+0) indicating that EG was protected by the substrate to a certain extent during denaturation. Comparison with the results from fluorescence emission spectroscopy revealed that the inactivation of EG occurred before the marked conformational changes could be detected.zh_CN
dc.description.sponsorshipNational Foundation for Fostering Talents of Basic Science[J1030626]; National Key Basic Research Program (NKBRP)[2010CB732201]; Natural Science Foundation of Fujian Province[2009J01196]zh_CN
dc.language.isoenzh_CN
dc.publisherAMER CHEMICAL SOCzh_CN
dc.subjectcellulasezh_CN
dc.subjecthydrolysiszh_CN
dc.subjectendoglucanasezh_CN
dc.subjectdenaturationzh_CN
dc.subjectureazh_CN
dc.subjectAspergilluszh_CN
dc.titleCellulase Hydrolysis of Rice Straw and Inactivation of Endoglucanase in Urea Solutionzh_CN
dc.typeArticlezh_CN


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