• 中文
    • English
  • English 
    • 中文
    • English
  • Login
View Item 
  •   DSpace Home
  • 生命科学学院
  • 生命科学-已发表论文
  • View Item
  •   DSpace Home
  • 生命科学学院
  • 生命科学-已发表论文
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Cellulase Hydrolysis of Rice Straw and Inactivation of Endoglucanase in Urea Solution

Thumbnail
Full Text
Cellulase Hydrolysis of Rice Straw and Inactivation of Endoglucanase in Urea Solution.htm (392bytes)
Date
2011-09-15
Author
Tao, Yi-Ming
Xu, Xin-Qi
Ma, Su-Juan
Liang, Ge
Wu, Xiao-Bing
Long, Min-Nan
龙敏南
Chen, Qing-Xi
陈清西
Collections
  • 生命科学-已发表论文 [5901]
Show full item record
Abstract
In order to optimize the cellulase (from Aspergillus glaucus) hydrolysis of pretreated rice straw, the effects of varying enzyme concentration, temperature, and pH were studied. The best experimental conditions found to degrade the pretreated rice straws were 24 h of incubation at 55 degrees C and pH 5.0, with an enzyme concentration of 48 mg/L. Urea is one of the important nitrogen sources used in fungi culture, but it is also a denaturant. The model of denaturation of endoglucanase (EG) in urea solutions was established. The denaturation was a slow, reversible reaction. Determination of microscopic rate constants showed k(+0) > K'(+0) indicating that EG was protected by the substrate to a certain extent during denaturation. Comparison with the results from fluorescence emission spectroscopy revealed that the inactivation of EG occurred before the marked conformational changes could be detected.
Citation
J. Agric. Food Chem., 2011, 59 (20), pp 10971–10975
URI
http://dx.doi.org/doi:10.1021/jf203712n
WOS:000295915000026
https://dspace.xmu.edu.cn/handle/2288/11836

copyright © 2002-2016  Duraspace  Theme by @mire  厦门大学图书馆  
About | Policies
 

 

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

LoginRegister

copyright © 2002-2016  Duraspace  Theme by @mire  厦门大学图书馆  
About | Policies