Cellulase Hydrolysis of Rice Straw and Inactivation of Endoglucanase in Urea Solution
Date
2011-09-15Author
Tao, Yi-Ming
Xu, Xin-Qi
Ma, Su-Juan
Liang, Ge
Wu, Xiao-Bing
Long, Min-Nan
龙敏南
Chen, Qing-Xi
陈清西
Collections
- 生命科学-已发表论文 [5901]
Abstract
In order to optimize the cellulase (from Aspergillus glaucus) hydrolysis of pretreated rice straw, the effects of varying enzyme concentration, temperature, and pH were studied. The best experimental conditions found to degrade the pretreated rice straws were 24 h of incubation at 55 degrees C and pH 5.0, with an enzyme concentration of 48 mg/L. Urea is one of the important nitrogen sources used in fungi culture, but it is also a denaturant. The model of denaturation of endoglucanase (EG) in urea solutions was established. The denaturation was a slow, reversible reaction. Determination of microscopic rate constants showed k(+0) > K'(+0) indicating that EG was protected by the substrate to a certain extent during denaturation. Comparison with the results from fluorescence emission spectroscopy revealed that the inactivation of EG occurred before the marked conformational changes could be detected.
Citation
J. Agric. Food Chem., 2011, 59 (20), pp 10971–10975URI
http://dx.doi.org/doi:10.1021/jf203712nWOS:000295915000026
https://dspace.xmu.edu.cn/handle/2288/11836