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dc.contributor.authorLu, Chun-Hua(Fuzhou Univ, Coll Chem & Chem Engn)
dc.contributor.authorLi, Juan(Fuzhou Univ, Coll Chem & Chem Engn)
dc.contributor.authorQi, Xiu-Juan(Fuzhou Univ, Coll Chem & Chem Engn)
dc.contributor.authorSong, Xiao-Rong(Fuzhou Univ, Coll Chem & Chem Engn)
dc.contributor.authorYang, Huang-Hao(Fuzhou Univ, Coll Chem & Chem Engn)
dc.contributor.authorChen, Xi
dc.contributor.author陈曦
dc.contributor.authorChen, Guo-Nan(Fuzhou Univ, Coll Chem & Chem Engn)
dc.date.accessioned2012-03-22T01:15:11Z
dc.date.available2012-03-22T01:15:11Z
dc.date.issued2011-06-15
dc.identifier.citationJOURNAL OF MATERIALS CHEMISTRY,2011,21(29):10915-10919zh_CN
dc.identifier.issn0959-9428
dc.identifier.urihttp://dx.doi.org/doi:10.1039/c1jm11121c
dc.identifier.uriWOS:000292978600050
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/11833
dc.description.abstractIn this article, we present a new method for the multiplex detection of nucleases by using graphene oxide (GO) as a platform. We introduce a Y-shaped DNA (Y-DNA) as the multiplex probe. The 5' termini of the Y-DNA are labeled with carboxy fluorescein (FAM), 6-carboxy-X-rhodamine (ROX) and cyanine 5 (Cy5) and they include three nuclease cleavage sites corresponding to PvuII, EcoRV and HaeIII, respectively. Upon the addition of nucleases, the nucleases cleave the corresponding sites in Y-DNA. Then, short dsDNA fragments containing fluorophores were released from the Y-DNA. These dsDNA fragments were unstable and easy to unwind into two short ssDNAs. They were then adsorbed onto the GO surface. Because of the excellent electronic transference of GO, the fluorescence intensity of the fluorophores can be quenched efficiently. Therefore, by monitoring the fluorophores' fluorescence change before and after the addition of the nucleases, it is easy to establish a platform of a Y-DNA/GO complex for the simultaneous multiplex detection of nucleases.zh_CN
dc.description.sponsorshipNational Natural Science Foundation of China[20735002, 20975023]; National Basic Research Program of China[2010CB732403]; Program For New Century Excellent Talents In University of China[09-001 4]; National Science Foundation of Fujian Province[2010J06003]zh_CN
dc.language.isoenzh_CN
dc.publisherROYAL SOC CHEMISTRYzh_CN
dc.titleMultiplex detection of nucleases by a graphene-based platformzh_CN
dc.typeArticlezh_CN


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