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Multiplex detection of nucleases by a graphene-based platform

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Multiplex detection of nucleases by a graphene-based platform.pdf (231.6Kb)
Date
2011-06-15
Author
Lu, Chun-Hua(Fuzhou Univ, Coll Chem & Chem Engn)
Li, Juan(Fuzhou Univ, Coll Chem & Chem Engn)
Qi, Xiu-Juan(Fuzhou Univ, Coll Chem & Chem Engn)
Song, Xiao-Rong(Fuzhou Univ, Coll Chem & Chem Engn)
Yang, Huang-Hao(Fuzhou Univ, Coll Chem & Chem Engn)
Chen, Xi
陈曦
Chen, Guo-Nan(Fuzhou Univ, Coll Chem & Chem Engn)
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  • 化学化工-已发表论文 [14469]
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Abstract
In this article, we present a new method for the multiplex detection of nucleases by using graphene oxide (GO) as a platform. We introduce a Y-shaped DNA (Y-DNA) as the multiplex probe. The 5' termini of the Y-DNA are labeled with carboxy fluorescein (FAM), 6-carboxy-X-rhodamine (ROX) and cyanine 5 (Cy5) and they include three nuclease cleavage sites corresponding to PvuII, EcoRV and HaeIII, respectively. Upon the addition of nucleases, the nucleases cleave the corresponding sites in Y-DNA. Then, short dsDNA fragments containing fluorophores were released from the Y-DNA. These dsDNA fragments were unstable and easy to unwind into two short ssDNAs. They were then adsorbed onto the GO surface. Because of the excellent electronic transference of GO, the fluorescence intensity of the fluorophores can be quenched efficiently. Therefore, by monitoring the fluorophores' fluorescence change before and after the addition of the nucleases, it is easy to establish a platform of a Y-DNA/GO complex for the simultaneous multiplex detection of nucleases.
Citation
JOURNAL OF MATERIALS CHEMISTRY,2011,21(29):10915-10919
URI
http://dx.doi.org/doi:10.1039/c1jm11121c
WOS:000292978600050
https://dspace.xmu.edu.cn/handle/2288/11833

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