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dc.contributor.authorGao, Xiang
dc.contributor.authorChen, Weizhu(State Ocean Adm, Inst Oceanog 3)
dc.contributor.authorZhu, Gangtian
dc.contributor.authorYi, Ruizao(State Ocean Adm, Inst Oceanog 3)
dc.contributor.authorWu, Zhen
dc.contributor.authorXu, Pengxiang
dc.contributor.authorZhao, Yufen
dc.contributor.author赵玉芬
dc.date.accessioned2012-03-14T02:05:49Z
dc.date.available2012-03-14T02:05:49Z
dc.date.issued2011-01-22
dc.identifier.citationJOURNAL OF CHROMATOGRAPHY A,2011,1218(10):1416-1422zh_CN
dc.identifier.issn0021-9673
dc.identifier.urihttp://dx.doi.org/doi:10.1016/j.chroma.2011.01.046
dc.identifier.uriWOS:000288186900013
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/11743
dc.description.abstractThe nucleoside-based antiviral phosphoramidates and H-phosphonates were synthesized and separated using reversed-phase liquid chromatography on bridged ethane hybrid (BEH) C(18) column packed with 1.7 mu m particles of non-chiral stationary phase. The influences of the composition of mobile phase and column temperature have been investigated to optimize the diastereoisomeric separation. Complete separations of the phosphoramidate and H-phosphonate prodrugs with good resolution (R(s) = 1.99-2.77) were achieved within a short time (5-9 min). The validation study of the optimized method including linearity, accuracy, repeatability and detection limit has revealed it is better performance versus conventional HPLC method. In addition, HPLC was combined with high resolution electrospray ionization time-of-flight mass spectrometry (ESI-TOF MS), which enabled the exact mass measurement and high sensitivity. Using MS as detection, the limits of detection and limits of quantification of the studied pronucleotide diastereoisomers were determined in the range of several nmol L(-1) level. (C) 2011 Elsevier B.V. All rights reserved.zh_CN
dc.description.sponsorshipNational Natural Science Foundation of China[20972130, 20805037, 20732004]zh_CN
dc.language.isoenzh_CN
dc.publisherELSEVIER SCIENCE BVzh_CN
dc.subjectDiastereoisomer separationzh_CN
dc.subjectPhosphoramidatezh_CN
dc.subjectH-phosphonatezh_CN
dc.subjectReversed-phasezh_CN
dc.subjectHPLC-MSzh_CN
dc.titleFast separation of antiviral nucleoside phosphoramidate and H-phosphonate diastereoisomers by reversed-phase liquid chromatographyzh_CN
dc.typeArticlezh_CN


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