Anisakis pegreffii: A quantitative fluorescence PCR assay for detection in situ
- 生命科学－已发表论文 
To facilitate improved diagnosis and detection of the third stage larva (L3) of Anisakis pegreffii from the Minnan-Taiwan bank fishing ground in Taiwan Strait, a real-time PCR method for the detection in situ and differentiation was developed to amplify a region of the second internal transcribed spacer (ITS-2) of this parasite. The real-time PCR assay was capable of detecting 1/3 of a single 13 in 30 mg of marine fish tissue, and also exhibited a high level of specificity for A. pegreffii, no fluorescence signals were observed in other five major larval anisakid species found in commercial marine fishes caught in this fishing ground. (C) 2010 Elsevier Inc. All rights reserved.
CitationExperimental Parasitology 2011,Vol.127 Issue(2):587-592