EDTA-Assisted Self-Assembly of Fluoride-Substituted Hydroxyapatite Coating on Enamel Substrate
- 化学化工－已发表论文 
Reconstructed layers containing ordered enamel-like structures of fluoride-substituted hydroxyapatite (FHAp) microcrystals were constructed on a human enamel surface using ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA) as the mediating agent under near-physiological conditions (pH 6.00, 37 degrees C, 1 atm). The effects of initial pH value, fluoride concentration, as well as reaction time on the formation of the FHAp microcrystals, including their micro-architectural structure, crystalline phase, chemical components, and hardness properties, were investigated using transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and Vickers microhardness measurements. The results of these in vitro experiments indicated that EDTA induced the assembly of hexagonal prismlike FHAp microcrystals along their c-axis direction, and the microcrystals further amalgamated with the extended reaction time. In addition, fluoride ions were found to play a critical role in the formation of hexagonal FHAp microcrystals. Interestingly, after reaction for 5 days, the Vickers microhardness of the new layer (347-370 VHN) was harder than that of natural tooth. On the basis of the experimental evidence, a mesoscale self-assembly mechanism was proposed to explain the growth of FHAp microcrystals.
CitationCRYSTAL GROWTH & DESIGN，2011,11（12）：5206-5214