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dc.contributor.authorChangqing Zhu
dc.contributor.authorShujuan Zhuo
dc.contributor.authorHong Zheng
dc.contributor.author郑洪
dc.contributor.authorJinlong Chen
dc.contributor.authorDonghui Li
dc.contributor.authorShunhua Li
dc.contributor.author李顺华
dc.contributor.authorJingou Xu
dc.contributor.author许金钩
dc.date.accessioned2011-12-07T00:48:44Z
dc.date.available2011-12-07T00:48:44Z
dc.date.issued2004
dc.identifier.citationMICROCHIMICA ACTA,2004,148(3-4):251-257zh_CN
dc.identifier.issn1436-5073
dc.identifier.urihttp://dx.doi.org/doi:10.1007/s00604-004-0268-5
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/11343
dc.description.abstractA near-infrared (near-IR) fluorescence recovery method for the determination of nucleic acids is presented. This method employs a two-reagent system composed of anionic heptamethines cyanine (HMC) and polycationic poly-lysine. The fluorescence of HMC, with maximum excitation and emission wavelengths at 778 and 804 nm, respectively, was quenched by poly-lysine in proper concentration, but recovered by adding nucleic acids. Under optimal conditions, the recovered fluorescence was proportional to the concentration of nucleic acids. The calibration graphs are linear over the range of 5-300 ng mL(-1) for herring sperm DNA (FS DNA), 2-100 ng mL(-1) for calf thymus DNA (CT DNA) and 5-500 ng mL(-1) for snake ovum RNA (SO RNA). The corresponding detection limits are 1.49 ng mL(-1) for FS DNA, 0.7 ng mL(-1) for CT DNA and 1.61 ng mL(-1) for SO RNA, respectively. Four synthetic and three real nucleic acid samples were determined with satisfactory results.zh_CN
dc.language.isoenzh_CN
dc.publisherSPRINGER WIENzh_CN
dc.subjectnear-IR fluorescencezh_CN
dc.subjectcyaninezh_CN
dc.subjectpoly-lysinezh_CN
dc.subjectnucleic acidszh_CN
dc.titleDetermination of nucleic acids based on shifting the association equilibrium between a heptamethine cyanine dye and poly-lysinezh_CN
dc.typeArticlezh_CN


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