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dc.contributor.authorLi, Qinxi
dc.contributor.author李勤喜
dc.contributor.authorYe, Zhiyun
dc.contributor.author叶志云
dc.contributor.authorWen, Jun
dc.contributor.authorMa, Lin
dc.contributor.authorHe, Ying
dc.contributor.authorLian, Guili
dc.contributor.authorWang, Zhen
dc.contributor.authorWei, Luyao
dc.contributor.authorWu, Di
dc.contributor.authorJiang, Bin
dc.date.accessioned2011-12-05T08:59:24Z
dc.date.available2011-12-05T08:59:24Z
dc.date.issued2009
dc.identifier.citationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,Volume 385, Issue 2, 24 July 2009, Pages 284-289zh_CN
dc.identifier.issn0006-291X
dc.identifier.urihttp://dx.doi.org/doi:10.1016/j.bbrc.2009.05.078
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/11302
dc.description.abstractThe tumor necrosis factor (TNF) can induce apoptosis in many cells including MCF-7 cells. To identify the genes responsible for TNF-induced apoptosis, we generated a series of TNF-resistant MCF-7 cell lines by employing restrovirus insertion-mediated random mutagenesis. In one of the resistant lines, gelsolin was found to be disrupted by vital insertion. Exogenous expression of gelsolin in this mutant cell line (Gel(mut)) restored the sensitivity to TNF-induced cell death and knock-down of gelsolin by siRNA conferred MCF-7 cells with resistance to TNF, indicating that gelsolin is required for TNF-induced apoptosis. Interestingly, the resistance of Gel(mut) cells to apoptosis induction is selective to TNF, since Gel(mut) and wild-type cells showed similar sensitivity to other death stimuli that were tested. Furthermore, TNF-induced ROS production in Gel(mut) cells was significantly decreased, demonstrating that gelsolin-mediated ROS generation plays a crucial role in TNF-induced apoptosis in MCF-7 cells. Importantly, caspase-mediated gelsolin cleavage is dispensable for TNF-triggered ROS production and subsequent apoptosis of MCF-7 cells. Our study thus provides genetic evidence linking gelsolin-mediated ROS production to TNF-induced cell death. (C) 2009 Elsevier Inc. All rights reserved.zh_CN
dc.description.sponsorship973 Program [2007CB914602, 2006CB503900]; National Natural Science Foundation of China [30770454, 30730025]; NCETXMUzh_CN
dc.language.isoenzh_CN
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCEzh_CN
dc.subjectGelsolinzh_CN
dc.subjectTNF-alphazh_CN
dc.subjectApoptosiszh_CN
dc.subjectROSzh_CN
dc.subjectMCF-7zh_CN
dc.titleGelsolin, but not its cleavage, is required for TNF-induced ROS generation and apoptosis in MCF-7 cellszh_CN
dc.typeArticlezh_CN


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