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dc.contributor.authorHan, Peng
dc.contributor.authorKang, Jin-He
dc.contributor.authorLi, Hua-Liang
dc.contributor.authorHu, Su-Xian
dc.contributor.authorLian, Hui-Hui
dc.contributor.authorQiu, Ping-Ping
dc.contributor.authorZhang, Jian
dc.contributor.authorLi, Wen-Gang
dc.contributor.authorChen, Qing-Xi
dc.contributor.author陈清西
dc.date.accessioned2011-12-05T08:52:45Z
dc.date.available2011-12-05T08:52:45Z
dc.date.issued2009
dc.identifier.citationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,Volume 385, Issue 2, 24 July 2009, Pages 251-256zh_CN
dc.identifier.issn0006-291X
dc.identifier.urihttp://dx.doi.org/doi:10.1016/j.bbrc.2009.05.059
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/11301
dc.description.abstractTamoxifen (TAM) is a nonsteroidal antiestrogen that has been used in the treatment of breast cancer for over 30 years. Recently, it was shown that TAM also has efficacy on gastrointestinal neoplasms such as hepatocarcinoma and pancreatic carcinoma, and that the chemopreventive activities of TAM might be due to its abilities to inhibit cell growth and induce apoptosis. In the present study, we investigated the effects of tamoxifen on growth and apoptosis in the human bile duct carcinoma (BDC cell line QBC939 using MTT assay, inverted microscopy, fluorescence microscopy, transmission election microscopy. classic DNA fragmentation agarose gel electrophoresis assay, PI single- and FITC/PI double-staining flow cytometry, and Western blotting. Our data revealed that TAM could significantly inhibit growth and induce apoptosis in QBC939 cells. Increased expression of p53 was observed in TAM-treated cells, indicating that p53 might play an important role in TAM-induced apoptosis in QBC939 cells. These results provide significant insight into the anticarcinogenic action of TAM on BDC. (C) 2009 Elsevier Inc. All rights reserved.zh_CN
dc.description.sponsorshipMedical Innovation Foundation of Fujian Province [2007-CXB-9]; Health Bureau Project of Xiamen [WSK0602]; Program for innovative Research Team in Science and Technology in Fujian Province Universityzh_CN
dc.language.isoenzh_CN
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCEzh_CN
dc.subjectTamoxifenzh_CN
dc.subjectBile duct carcinomazh_CN
dc.subjectAntiproliferationzh_CN
dc.subjectApoptosiszh_CN
dc.subjectp53zh_CN
dc.titleAntiproliferation and apoptosis induced by tamoxifen in human bile duct carcinoma QBC939 cells via upregulated p53 expressionzh_CN
dc.typeArticlezh_CN


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