Show simple item record

dc.contributor.authorJin-Can Shen
dc.contributor.authorSu-Ge Lu
dc.contributor.authorZhi-Xia Zhuang
dc.contributor.author庄峙厦
dc.contributor.authorXiao-Ru Wang
dc.contributor.author王小如
dc.contributor.authorFrank S.C. Lee
dc.date.accessioned2011-11-18T13:32:07Z
dc.date.available2011-11-18T13:32:07Z
dc.date.issued2005-03
dc.identifier.citationINTERNATIONAL JOURNAL OF MASS SPECTROMETRY,2005,243(2):163-169zh_CN
dc.identifier.issn1387-3806
dc.identifier.urihttp://dx.doi.org/doi:10.1016/j.ijms.2005.02.006
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/11202
dc.description.abstractAlthough positive-ion (PI) electrospray ionisation mass spectrometry (ESI-MS) has been usually applied for the analysis of native metallothioneins (MT) isoforms binding with metal ions, it suffers from the lack of insensitivity under neural conditions because of the low efficiency of protonation during ESI process. In this study, multiply deprotonated metallothionein (Zn-7-MT-2a), produced from tris(hydroxymethyl)aminomethane (TRIS) acetate solutions under near neutral condition, was analyzed by negative-ion (NI) ESI-MS. Compared with the ammonium acetate buffer system which has been normally used for ESI-MS experiments, the use of TRIS acetate buffer results in the formation of more abundant ions and higher charge states of MT-2a, and consequently higher intensity is attained. The sensitivity enhancement of the system could be explained by the high gas-phase proton affinity, small molecular volume of the anion (acetate), and the high hydrogen consumption by TRIS in the buffer system, which would all favor the deprotonation of the analyte during NI-ESI process. Factors that may affect the NI-ESI responses, such as acid added in buffer, pH and concentration of TRIS acetate had been evaluated, and the result showed that using acetic acid, lower pH under the pH range evaluated, higher concentration of TRIS acetate all favored the MS detection of MT-2a. Our finding sheds light on a buffer system that may offer substantial sensitivity advantages in the studying of weakly bound, non-covalent complexes such as metal binding MTs, which are usually analyzed under near neutral conditions. © 2005 Elsevier B.V. All rights reserved.zh_CN
dc.language.isoenzh_CN
dc.publisherELSEVIER SCIENCE BVzh_CN
dc.subjectpositive-ionzh_CN
dc.subjectnegative-ionzh_CN
dc.subjectelectrospray ionization mass spectrometryzh_CN
dc.subjecttris(hydroxymethyl)aminomethanezh_CN
dc.subjectmetallothioneinzh_CN
dc.titleA sensitive negative-ion electrospray ionization mass spectrometry detection for metallothionein in tris(hydroxymethyl)aminomethane acetate bufferzh_CN
dc.typeArticlezh_CN


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record