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dc.contributor.authorYing Lai
dc.contributor.authorQiuquan Wang
dc.contributor.authorWenwu Yan
dc.contributor.authorLimin Yang
dc.contributor.author杨利民
dc.contributor.authorBenli Huang
dc.contributor.author黄本立
dc.date.accessioned2011-11-10T14:00:51Z
dc.date.available2011-11-10T14:00:51Z
dc.date.issued2005-06
dc.identifier.citationJ. Anal. At. Spectrom., 2005, 20(8): 751-753zh_CN
dc.identifier.issn0267-9477
dc.identifier.urihttp://dx.doi.org/doi:10.1039/B501766A
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/11157
dc.description.abstractConcentrations of rare earth elements (REEs) were determined in the laminae of 10 species of ferns and their acetone-extractable pigments, as well as their host soil and soil extract, by ICP-MS. A new REE hyperaccumulator, Pronephrium simplex, was discovered which could accumulate REEs up to 1.2 mg g(-1) dry mass under natural growth conditions. Three typical species of ferns chosen were divided into lamina, petiole, stem and root for the study of REE translocation and fractionation. A hyphenated technique, size exclusion HPLC coupled with online UV/ICP-MS, was developed to provide reliable evidence of the existence of REE-binding proteins in the fern's lamina. A new REE-binding protein was discovered and separated from the lamina of natural grown P. simplex. Further characterization of the protein showed that its molecular mass is 5068.4 Da by MALDI-TOF-MS and ESI-MS. Amino acid composition analysis by RP-HPLC indicated that the protein has relatively high contents of proline and glycin.zh_CN
dc.language.isoenzh_CN
dc.publisherROYAL SOC CHEMISTRYzh_CN
dc.titlePreliminary study of the enrichment and fractionation of REEs in a newly discovered REE hyperaccumulator Pronephrium simplex by SEC-ICP-MS and MALDI-TOF/ESI-MSzh_CN
dc.typeArticlezh_CN


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