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dc.contributor.authorZhu, CQ
dc.contributor.author朱昌青
dc.contributor.authorWu, YQ
dc.contributor.authorZheng, H
dc.contributor.author郑洪
dc.contributor.authorChen, JL
dc.contributor.authorLi, DH
dc.contributor.author李东辉
dc.contributor.authorLi, SH
dc.contributor.authorXu, JG
dc.date.accessioned2011-11-01T01:39:32Z
dc.date.available2011-11-01T01:39:32Z
dc.date.issued2004
dc.identifier.citationANALYTICAL LETTERS,2004, vol. 37, no6, pp. 1115-1127zh_CN
dc.identifier.issn0003-2719
dc.identifier.urihttp://dx.doi.org/doi:10.1081/AL-120034057
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/11106
dc.description.abstractA new method based on near-infrared (near-IR) fluorescence recovery, employing a two-reagent system, which is composed of a cationic heptamethylene cyanine and poly-glutamate, is presented for the determination of protein. The fluorescence of cationic heptamethylene cyanine, with the maximum excitation and emission wavelengths at 800 and 815 urn, respectively, was quenched by poly-glutamate with a proper concentration, but recovered by adding proteins at pH 3.5. Under optimum conditions, the recovered fluorescence was in proportional to the concentration of proteins. The linear ranges of the calibration curves were 50-1000, 100-1500 and 100-1000ng/mL with the detection limit of 37, 40, 43 ng/mL for BSA, HSA, and gamma-IgG, respectively. The relative standard deviation (n = 8) was 1.7% for 400 ng/mL bovine serum albumin (BSA). The proposed method was applied to the determination of proteins in real serum samples with satisfactory results.zh_CN
dc.language.isoenzh_CN
dc.publisherMARCEL DEKKER INCzh_CN
dc.subjectcyaninezh_CN
dc.subjectpoly-glutamatezh_CN
dc.subjectproteinzh_CN
dc.subjection association equilibriumzh_CN
dc.subjectfluorometryzh_CN
dc.titleNear-infrared fluorometric determination of protein by shifting the ion-association equilibrium between cationic heptamethylene cyanine and poly-glutamatezh_CN
dc.typeArticlezh_CN


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