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dc.contributor.authorZhang, XB
dc.contributor.authorXu, X
dc.contributor.authorLi, QG
dc.contributor.author李庆阁
dc.contributor.authorXu, LM
dc.date.accessioned2011-10-31T10:19:05Z
dc.date.available2011-10-31T10:19:05Z
dc.date.issued2000
dc.identifier.citationPROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,vol27,issue3,pp227-280zh_CN
dc.identifier.issn1000-3282
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/11090
dc.description.abstractA molecular beacon probe was used in the detection of prawn white spot bacilliform virus (WSBV). The probe possesses a stem and loop structure. The sequence of loop is complementary to the WSBV DNA, and the stem is formed by two complementary arm sequences which are unrelated to the WSBV DNA. The probe underwent a fluorogenic conformational change as it hybridized to the WSBV DNA. The probe could not affect the amplification, and was specific and sensitive when used in PCR. It was showed that the fluorescent intensities increased as the cycles of PCR and the number of target DNA copy increased.zh_CN
dc.language.isoenzh_CN
dc.publisherSCIENCE PRESSzh_CN
dc.subjectprawn white spot bacilliform viruszh_CN
dc.subjectpolymerase chain reaction (PCR)zh_CN
dc.subjectmolecular beacon probezh_CN
dc.titlePCR detection of prawn white spot bacilliform virus (WSBV) using molecular beacon probe.zh_CN
dc.typeArticlezh_CN


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